Usages:
For selective isolation of Gram-negative bacteria, especially for Shigella and Salmonella.
Item Code: HCM012
Packaging: 500g
Principle:
Yeast extract powder provides nitrogen, vitamins, growth factors; sodium chloride to maintain osmotic equilibrium ferric ammonium citrate of iron salts to produce a black iron sulfide; agar as medium coagulant; phenol red as pH indicator.
Formulation?per litre?:
Xylose 3.50g
L-Lysine 5.00g
Lactose 7.50g
Sucrose 7.50 g
Sodium Chloride 5.00 g
Yeast Extract 3.00g
Sodium Desoxycholate 2.50g
Sodium Thiosulphate 6.80g
Fe-Ammonium Citrate 0.80g
Phenol Red 0.08g
Agar 13.50
Final PH 7.4±0.2
How to use:
- Suspend 55.2g in 1Lof distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Do not autoclave, cool to 50oC and pour into sterile Petri dishes.
- Diluted and treated samples.
Quality control:
Quality control strains were inoculated, and cultured at 36 ± 1oC for 24h, results show as follows:
| Item |
The name and number of strain |
Growth |
Colony Color |
| 1 |
Arizona bacteria CMCC (B) 47001 |
Good |
Black colonies |
| 2 |
Salmonella typhimurium CMCC (B) 50115 |
Good |
Black colonies |
| 3 |
Streptococcus faecalis CMCC32223 |
Prohibited |
-- |
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
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MalaysiaMY
IndonesiaID
MyanmarMM
