Vogel Johnson Agar

Item Code: HCM046
Packaging: 500g
 
Usages:
For selective isolation of staphylococcus aureus.
 
Principle:
Peptone and beef extract powder provide carbon, nitrogen, vitamins and minerals; D- mannitol to fermentable sugars; higher levels of sodium chloride to provide a higher osmotic pressure, suppress most non-staphylococcal microorganisms; phenolsulfonphthalein as pH indicator; agar is a medium coagulant. Typical pathogenic staphylococci (coagulase-positive) D- mannitol produces acid fermentation and produces yellow colonies with a yellow halo, typically non-pathogenic Staphylococcus unfermented D- mannitol to form red colonies.
 
Formulation?per litre ?:

Pancreatic Digest of Casein	10g
Yeast Extract	5 g
Mannitol	10 g
Dibasic Potassium Phosphate	5 g
Lithium chloride	5 g
Glycine	10 g
Agar	16 g
Phenol Red	25mg
Final pH	7.2±0.2

 
How to use?
1. Suspend 61g in 1L of distilled water, stirring heated to boiling to completely dissolve, autoclave at 121? for 15 minutes.
2. Diluted and treated samples.
 
Quality control?
          ??

Item	The name and number of strain	Growth	Colony Color
1	Staphylococcus aureus CMCC (B) 26003	Good	Golden yellow
2	Staphylococcus epidermidis CMCC (B) 26069	Good	Red
3	Escherichia coli CMCC (B) 44102	Inhibition	--

 
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.