Item code: SN016-0100
Size: 100 Reactions
Sample:
Up to 200 μl of virus sample
Format: Reagent and mini spin column
Sample material: Serum, plasma, body fluids
Operation time: 20 minutes
Elution volume: 50 μl
Feature
- High binding capacity for viral RNA or viral DNA.
Kit Contents
Contents |
Size |
Buffer V1 |
45 ml |
Buffer V2 (Add ethanol) |
6 ml (45 ml) |
Buffer W1 |
45 ml |
Buffer W2 (Add ethanol) |
15 ml |
Buffer RE |
10 ml |
VN Columns |
50 pieces x 2 |
Collection Tube |
50 pieces x 2 |
The Virus Nucleic Acid Isolation Kit provides a fast, simple, and cost-effective method for the isolation of viral DNA/RNA from cell-free samples such as serum, plasma, body fluids and the supernatant of virus-infected cell cultures. Its unique buffer system will eff iciently lyse cells and degrade proteins, allowing for the nucleic acid to be easily bound by the glass f iber matrix of the column. Contaminants such as salts, metabolites and soluble macromolecular cellular components are removed in the Wash Step. The phenol extraction and ethanol precipitation are not required, and the high-quality nucleic acid is eluted in the RNase-free elution buffer. The viral DNA/RNA i solated with the Total Nucleic Acid Isolation Kit (Virus) is suitable for a variety of routine applications, including the real-time PCR/RT-PCR, automated fluorescent DNA sequencing, PCR, and other enzymatic reactions. The entire procedure can be completed within 15-20 minutes.
Applications
- Real-time PCR.
- RT-PCR.
- DNA sequencing.
- PCR.
- Enzymatic reactions.
Quality Control
The quality of the Virus Nucleic Acid Isolation Kit is tested on a lot-to-lot basis to ensure consistent product quality.