Usages:
For selective isolation of Enterobacter sakazakii and differentiating of enterobacteriaceae.
Principle:
Peptone and yeast extract powder provides carbon and nitrogen sources and traces elements; lactose is fermentable sugars; sodium chloride maintains osmotic equilibrium; the 3rd bile salts and crystal violet inhibiting gram-positive bacteria, especially against Gram-positive bacteria and faecal streptococci; neutral red as pH indicator.
Item Code: HCM006
Packaging size: 500g
Formulation?per litre):
Pancreatic Digest of Gelatin: 7g
Yeast extract powder: 3g
Lactose: 10g
Glucose Monohydrate: 10g
Sodium chloride: 5g
The 3rd bile: 1.5g
Neutral Red :0.03g
Crystal violet: 0.002g
Agar: 15g
Final pH 7.4 ± 0.2
How to use?
1. Suspend 41.5g in 1 L of distilled water, stirring heated to boiling until completely dissolved, dispensing flask, autoclave at 121oC for 15min.
2. Diluted and treated samples.
Quality control?
| Item |
The name and number of strain |
Growth |
Colony Color |
| 1 |
Escherichia coli ATCC25922 |
Good |
Dark red |
| 2 |
Aerogenes CMCC (B) 45103 |
Good |
Red |
| 3 |
Staphylococcus aureus ATCC6538 |
Suppressed |
-- |
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
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IndonesiaID
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