Violet Red Bile Agar

Item Code: HCM071
Packaging: 500g
 
Usages:
For selective isolation of Enterobacteriaceae.
 
Principle:
Peptone and yeast extract powder provides carbon and nitrogen sources and traces elements; lactose is fermentable sugars; sodium chloride maintains osmotic equilibrium; the 3rd bile salts and crystal violet inhibiting gram-positive bacteria, especially against Gram-positive bacteria and faecal streptococci; neutral red as pH indicator.
 
Formulation?per liter?:
Yeast extract powder 3g
Pancreatic digest of gelatin 7g
Bile salt 1.5g
Lactose 10g
Sodium chloride 5g
D-Glucose Monohydrate 10g
Neutral Red 0.03g
Crystal violet 0.002g
Agar 15g
Final pH 7.4 ± 0.2          
 
How to use?
1. Suspend 51.5g in 1 L of distilled water, stirring heated to boiling until completely dissolved, Do not autoclave.
2. Diluted and treated samples.
 
Quality control?
          ??

Item	The name and number of strain	Growth	Colony Color
1	Escherichia coli ATCC25922	Good	Dark red
2	Aerogenes CMCC (B) 45103	Good	red
3	Staphylococcus aureus ATCC6538	Suppressed	--

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.