Triple Sugar Iron Agar

HCM014 Triple Sugar Iron Agar (TSI)
 
Usages:
For differentiating Gram-negative enteric bacilli.

Item code: HCM014 
Packaging: 500g
 
Principle:
Peptone, beef extract powder provide nitrogen, vitamins, minerals; lactose, dextrose, sucrose to fermentable sugars, which produce acid when measured by the phenol red indicator, acid yellow, basic red; sodium chloride to maintain osmotic equilibrium; agar as medium coagulant.
 
Formulation (per liter):
Pancreatic Digest of Casein: 10g
Pancreatic Digest of Animal Tissue: 10g
Lactose 10g Sucrose 10g Dextrose: 1g
Ferrous Ammonium Sulfate: 200mg
Sodium Chloride: 5g
Sodium Thiosulfate: 200mg
Agar: 13g
Phenol Red: 25mg
Final pH: 7.3±02
 
How to use:

1. Suspend 59.4g in 1L of distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121? for 15 minutes. Cool a slanted position.
2. Diluted and treated samples.

 
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.