Stable Cell Lines

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ACE2 and Spike Protein Stable Cell Lines

GenScript has developed single clone stable cell lines with high expression of the key regulators of COVID19, SARS-CoV-2 Spike protein or human ACE2 (hACE2), and two options of host cells – HEK293 or CHO-K1, as powerful cell line tools for drug discovery to treat COVID19.

Both HEK293/ACE2 and CHO-K1/ACE2 stable cell lines are generated with the features below:

• Single clone cell line
• Validated for stable hACE2 expression and S protein RBD binding
• Easy to use in binding assays or as immunogens

Applications:

• Neutralization assays to screen antibodies or other molecules that can block pseudovirus or live virus infection
• Affinity analysis of antibody or other molecules to Spike protein
• In vitro cell models for investigating SARS-CoV-2 infection mechanism

Immune Checkpoint Stable Cell Lines

Inhibitors of immune-checkpoint proteins, such as PD1, PD-L1, VISTA, Tim3 and Lag3, have indicated broad and diverse opportunities to increase antitumor immunity by enhancing T cell activity with the potential to produce durable clinical responses. To meet these needs, GenScript has developed stable cell line products expressing the most popular immune checkpoints to accelerate your immunotherapy discovery and clinical translation. All these stable cells are developed for screening high affinity antibodies against immune checkpoint proteins.

Key features:

• High protein expression at the cell surface validated by FACS
• Stable cell lines for increased reliability and reproducibility (over 15 passages)
• Ready to use hot targets, saving 3-5 months

Fc Receptor Stable Cell Lines

Fc receptor(FcR) types are classified based on the antibodies that they recognize. Different antibody subclasses have unique affinities for each of the Fc receptors. GenScript offers a full portfolio of Fc receptor stable cell lines to help you develop in vitro assays and characterize your antibodies, such as antibody binding, affinity, and half-life time test.

GPCR Stable Cell Lines

GenScript offers a wide selection of GPCR stable cell lines. Proprietary codon optimization and vector design technologies enable GenScript to overcome low functional protein expression at the cell surface. All of the cell lines have been optimized for S/B ratio in calcium flux assays, FRET assays, or chemiluminescent assays.

Key features:

• Gα15 expressing cells can be used directly for FLIPR analysis
• Stable cell lines for increased reliability and reproducibility
• Flexible functional readouts are available