Simmons Citrate Agar

Item Code: HCM109
Packaging: 500g
 
Usages:
For the differentiation of Gram-negative bacteria on the basis of citrate utilization.
 
Principle:
Magnesium ions in various metabolic cofactors; ammonium dihydrogen phosphate to provide nitrogen; dipotassium hydrogen phosphate is the buffer; sodium citrate as a carbon source; agar as medium coagulant.
 
Formulation?per liter?:
Sodium chloride 5g
Magnesium sulfate 0.2g
Ammonium dihydrogen phosphate 0.2g
Sodium ammonium phosphate 0.8g
Sodium citrate 2g
Agar 15g
Bromothymol blue 0.08g
Final pH 7.0 ± 0.2
 
How to use?
1. Suspend 23.3g in 1 L of distilled water, stirring heated to boiling until completely dissolved, dispensing flask, 121 ? autoclaves for 15min.
2. Diluted and treated samples.
 
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.