Opal 7-Color Automation IHC Kit 50 Slide. Opal™ is a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image. The method is similar to standard immunohistochemistry (IHC).
Users should source primary antibodies and other workflow reagents separately. The Opal 7-Color Automation IHC Kit was optimized for use on the Leica Biosystems BOND RX and configured and validated for imaging on the Vectra® and Mantra™ systems. Kits contain enough reagents to stain 50-slides at the recommended 1:150 dilution ratio.
Opal will enable you to:
Measure up to six tissue biomarkers at once
Use the best primary antibodies together in multiplex panel, with no species-based crosstalk
Retain spatial cellular context that is lost in flow cytometry
Confirm single cell co-expression for many biomarkers in one tissue section
Get more information while conserving precious tissue.
Automating Opal staining brings the benefit of:
Quality, consistency and reproducibility with every sample
Time-savings versus laborious manual process
Ease-of-use with a walk-away, high-throughput protocol