Random Hexamers primer

Item code: SM702-0100

Conc.: 50uM
Size: 100ul
Store at -20°C

Random Hexamers are short oligodeoxyribonucleotides of random sequences [d(N)6] that anneal to random complementary sites on a target DNA or RNA and serve as primers for DNA synthesis by a DNA polymerase or reverse transcriptase.
During cDNA generation, random priming gives random coverage to all regions of the RNA to generate a cDNA pool containing various lengths of cDNA. Random priming is incapable of distinguishing between the mRNA and other RNA species present in the reaction.

Protocol
We recommend using 1 μl of Random Hexamers primer per 20 μl reaction volume.

Quality control
This product is qualified in a cDNA reaction.

Applications

• cDNA synthesis using a Reverse Transcriptase with RNA templates.
• DNA synthesis using the Klenow fragment with DNA templates.
• DNA probe synthesis for use in Southern, Northern, and in situ hybridization applications.

Notes
For research use only.