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Plasmid miniPREP Kit

The Plasmid miniPREP Kit provides a fast, simple, and cost-effective method for the plasmid DNA isolation from the cultured bacterial cells.

Pricing & Availability

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Size: 100 Reactions / 300 Reactions

Features

Ready-to-use DNA for high performance in any downstream application.
Consistent DNA yields from a small amount of the starting material.
Time flexibility.

Kit Contents

Contents	300 Reactions	100 Reactions
Buffer S1	65 ml	25 ml
Buffer S2	65 ml	25 ml
Buffer S3	95 ml	35 ml
Buffer W1	125 ml	45 ml
Buffer W2 (Add ethanol)	25 ml x 2 (100 ml X 2)	15 ml x 1 (60 ml X 1)
Buffer BE	30 ml	10 ml
RNase A (50mg/mL)	150 μl	50 μl
PM Column	50 pieces x 6	50 pieces x 2
Collection Tube	50 pieces x 6	50 pieces x 2

The Plasmid miniPREP Kit provides a fast, simple, and cost-effective method for the plasmid DNA isolation from the cultured bacterial cells. The Plasmid miniPREP Kit is based on the alkaline lysis of bacterial cells, followed by the binding of the DNA onto the glass fiber matrix of the spin column in the presence of high salt. The phenol extraction and ethanol precipitation are not required, and the high-quality plasmid DNA is eluted in a small volume of Tris buffer (included in the kit) or water (pH between 7.0 and 8.5, not provided in the kit). The plasmid DNA purified with the Plasmid miniPREP Kit is suitable for a variety of routine applications including restriction enzyme digestion, sequencing, library screening, in vitro translation, transfection of robust cells, ligation, and transformation. The entire procedure can be completed within 15-20 minutes.