OnePCR™

 OnePCR™

Item code: SM203-0100

Size: 100 Reactions (2 X 1.25 ml)

Features

• No post-staining processing of DNA required.
• No need to prepare PCR Reagents.
• Direct loading onto your agarose gel for analysis.
• Sensitivity – High degree of sensitivity as the ethium bromide.
• Speed – No destaining requirement.
• Compatibility – Use the blue light or UV to detect the signal.

OnePCR™ is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. OnePCR™ is a pre-mixed solution containing Taq DNA polymerase, PCR Buffer, dNTP, gel loading dyes, and fluorescence dye.OnePCR™ which contains the Taq DNA polymerase, is purified from the E. coli., and expressing the Thermus aquaticus DNA polymerase gene. This enzyme has a 5′ → 3′ DNA polymerase and the 5′ → 3′ exonuclease activity but lacks the 3′ → 5′ exonuclease activity. OnePCR™, which contains the fluorescence dye, is directly detected on BLooK LED transilluminator or UV epi-illuminator after the DNA electrophoresis. OnePCR™ mixture is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions.Reagents are provided with the sufficient amplification reactions of 50 μl each.

Fig.1

Fig.1 OnePCR products are viewed using the Blue Light (left) or UV (right) to detect the signal.
Exposure to the Blue Light (left gel), and UV light (right gel)
Lane M: 1 kb Marker
Lanes 1~3: 1 kb, lanes 4~6: 2 kb, lanes 7~9: 4 kb
Template: p35s-GFP

Fig. 2

Fig. 2 PCR products of the genomic DNA (lane 1) or cDNA (lane 2) of Rose petal.
Exposure to the Blue Light.
Primer: Actin Forward Primer / Actin Reverse Primer
Lane M: 1 kb Marker

Fig. 3

Fig. 3 PCR products of the storage condition test for 3 months.
Exposure to the Blue Light.
M: 1 kb Marker
Lane 1: Room temperature for 3 months
Lane 2: 4°C for 3 months
Lane 3: -20°C for 3 months
Lane 4: 0 day

Fig. 4

Fig. 4 Sequence analysis . PCR products of both the general PCR(up) and OnePCR(down) from the p35s-GFP plasmid sequencing results exhibited as the same.

Fig. 5

Fig. 5 Results of the colony PCR separation on the 1.2% agarose gel.
Exposure to the UV light
Lane M: 1 kb Marker
Target: Lanes 1~3 : 1kb
Lanes 4~6: 4kb
Lanes 7~ 9 : 2kb
Template: p35S-GFP Plasmid

Fig. 6

Fig. 6 Cloning test of the OnePCR and general PCR products.After ligation , perform the PCR check on the insert.
Exposure to the Blue Light.
Insert: General PCR and OnePCR reaction products .
Primer : M13 Forward Primer / M13 Reverse Primer
Lane M: 1 kb Marker
Lane 1: General PCR
Lane 2: OnePCR

Storage
Store at RT up to 3 months
Store at 4°C up to 6 months
Store at -20°C up to 1 year
Shipping Temperature: 4°C
Note: OnePCR™ is light sensitive and should be stored and protected from light.

Application
?  No post-staining processing of DNA required.
?  No need to prepare PCR Reagents.
?  Direct loading onto your agarose gel for analysis.
?  Sensitivity – High degree of sensitivity as the ethium bromide.
?  Speed – No de-staining requirement.
?  Compatibility – Use the blue light or UV to detect the signal.

OnePCR™ HiFi

Item code: SM205-0100

Size: 100 Reactions (2 X 1.25 ml)

Features

• No post-staining procession.
• No need to prepare PCR reagents.
• Direct loading onto your agarose gel for analysis.
• Sensitivity- High degree of sensitivity as the ethium bromide.
• Time Efficiency- No destaining requirement.
• Compatibility – Use the Blue Light or UV to detect the signal.
• Effective for the amplification of GC-rich targets.
• Exhibits strong 3’→5′ exonuclease activity.

The OnePCR™ HiFi is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. The OnePCR™ HiFi is a pre-mixed solution containing GDP-HiFi DNA polymerase, PCR reaction buffers, dNTPs, gel loading dyes, enhancer, and fluorescence dye. The OnePCR™ HiFi contains the GDP-HiFi DNA polymerase which exhibits strong proof-reading activity, excellent processivity, and elongation capability. The elongation rate is 106-138 bases / second, approximately two times higher than the Taq DNA polymerase. The OnePCR™ HiFi also contains the fluorescence dye, which is directly detected on BLooK LED Transilluminator (BK001) or UV epi-illuminator after the DNA electrophoresis. The OnePCR™ HiFi mixture is supplied at the 2X concentration to allow 50% of the final reaction volume to be used for the addition of the primer and template solutions. Reagents are provided with sufficient amplification reactions of 50 μl. The OnePCR™ HiFi produces blunt end PCR products.

Storage
Store at room temperature up to 3 months
Store at 4°C up to 6 months
Store at -20°C up to 1 year
Shipping temperature: 4°C

OnePCR™ HotStar

Item code: SM206-0100

Size: 100 Reactions (2 X 1.25 ml)

Features

• No post-staining processing of DNA required.
• No need to prepare PCR Reagents.
• Direct loading onto your agarose gel for analysis.
• Sensitivity – High degree of sensitivity as the ethium bromide.
• Speed – No destaining requirement.
• Compatibility – Use the Blue Light or UV to detect the signal.
• Specificity – Reduce the non-specific amplification products, thus significantly improving the specificity of PCR reactions.

OnePCR™ HotStar is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. OnePCR™ HotStar is a pre-mixed solution containing Hot Start Taq DNA polymerase, PCR reation buffers, dNTPs, gel loading dyes, enhancer, and fluorescence dye. OnePCR™ HotStar contains Hot Start Taq DNA polymerasewhich exhibits non-template-dependent terminal transferase activity that adds a 3’ deoxyadenosine to product ends, and 5’→3’ exonuclease activity (but not 3’→5’ exonuclease activity). The enzyme contains a proprietary antibody that blocks polymerase activity at ambient temperatures. Activity is restored after the initial denaturation step in PCR cycling at 94°C, thereby providing an automatic “hot start” for Taq DNA polymerase in PCR. OnePCR™ HotStar also contains the fluorescence dye, which is directly detected on BLooK LED Transilluminator (BK001) or UV epi-illuminator after the DNA electrophoresis. OnePCR™ HotStar mixture is supplied at the 2X concentration to allow 50% of the final reaction volume to be used for the addition of primer and template solutions. Reagents are provided with sufficient amplification reactions of 50 μl each.

Storage
Store at room temperature up to 3 months
Store at 4°C up to 6 months
Store at -20°C up to 1 year
Shipping temperature: 4°C

BlooDireX PCR™ System

Item code: SM211-0100

Size: 100 Reactions

Features

• Sample is added directly to PCR reaction, therefore there is no need for time-consuming and expensive DNA purification steps.
• Master Mix format with premixed gel loading dye minimizes possibility of cross-contamination, reduces sample handling time and allows directly loading to gel.
• Amplify fragment size: up to 5 kilo base pairs.
• High speed: 15-30 seconds/ kilo base.

Kit Contents

The BlooDireX PCR™ System is designed for amplification of DNA from whole blood. It contains BlooDireX PCR and Novel Juice, suitable for PCR from blood sample directly. BlooDireX PCR™ is performed PCR directly from sample with no prior DNA extraction or sample preparation. For blood, stored at 4 °C or frozen, and preserved with EDTA, citrate or heparin are all suitable for this master mix. BlooDireX PCR™ is a premixed ready- to-use solution containing reaction buffers, dNTPs, loading dye, glycerol, PCR enhancers, and hot- start DNA polymerase. The hot-start taq prevents non-specific amplification due to mispriming and/or formation of primer dimers before thermal cycling, and it also exhibits 2X faster elongation rate than conventional Taq.

The modified hot-start DNA polymerase is resistant to most PCR inhibitors and retains its activity in the PCR reaction. BlooDireX PCR™ is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions. Reagents are provided with the sufficient amplification reactions of 20 μl each. Novel Juice is anon-mutagenic fluorescent reagent that produces instant visualization of DNA bands upon blue light (e.g. BLooK, BK001) or UV illumination of agarose gels. Supplied in 6X DNA Loading Buffer, Novel Juice is used to prepare DNA markers or samples for loading on agarose or polyacrylamide gels. Novel Juice is the most sensitive staining reagent available for detecting the double-stranded DNA (dsDNA). It contains three tracking dyes (bromophenol blue, xylene cyanol FF, and orange G) for visually tracking the DNA migration during the electrophoresis process. It is ideal for the environment requiring a safe, non-hazardous alternative to Ethidium Bromide (EtBr). Approximate fluorescence excitation / emission: 300, 495 / 537 nm, bound to nucleic acid.

Applications

• Genotyping.
• Transgenic detection.
• Gene knockout analysis.