Modified Phosphate Buffer

Item Code: HCM030
Packaging: 500g
     
Usages:
For enrichment of Yersinia bacteria.
 
Principle:
Sorbitol provides carbon and nitrogen sources, disodium hydrogen phosphate and sodium dihydrogen phosphate buffer solution, bile salts to inhibit gram-positive bacteria, sodium chloride provides osmotic cells need.
 
Formulation (per litre):
Disodium hydrogen phosphate 8.23g
Sodium dihydrogen phosphate (NaH2PO4.H2O) 1.2g
Sodium chloride 5.0g
The 3rd bile 1.5g
Sorbitol 20g
Final pH7.6
 
How to use:
1. Suspend 36g in 1 L of distilled or deionized water, heated to boiling stirring until completely dissolved, dispensing into a flask, autoclave at 121 ? for 15min.
2. Diluted and treated samples.
 
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.