MFC Agar Base

Item Code: HCM108
Packaging: 500g
 
Usages:
For testing of fecal coliform in the water with filter membrane method.
 
Principle:
Tryptone, polypeptone and yeast extract powder provide nitrogen, vitamins and growth factors; sodium chloride to maintain osmotic equilibrium; lactose to fermentable sugars; the 3rd bile suppresses gram-positive bacteria; agar as medium coagulant; Rose acid and aniline blue as a pH indicator.
 
Formulation?per liter?:
Tryptone 10g
Polypeptone 5g
Yeast extract powder 3g
Sodium chloride 5g
Lactose 12.5g
The 3rd bile 1.5g
Agar 15g
Rose Acid 0.1g
Aniline Blue 0.1g
Final pH7.4 ± 0.2
 
How to use?
1. Suspend 52.2g in 1 L of distilled water, stirring heated to boiling until completely dissolved, dispensing flask, 121 ? autoclaves for 15min.
2. Diluted and treated samples.
 
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.