Mannitol Yolk Polymyxin Agar Base

Item Code: HCM029 
Packaging: 500g
Supplement: SR0170  Polymyxin
     
Usages:
For determination and isolation of Bacillus cereus.
 
Principle:
Peptone and beef extract powder provide nitrogen, vitamins and growth factors; D- mannitol as fermentable sugars; sodium chloride to maintain osmotic equilibrium; agar as medium coagulant; phenol red as a pH indicator; yolk contains lecithin, lecithin to produce Bacillus cereus enzyme precipitation ring around the colonies; and D- mannitol produces acid fermentation Colonies was yellow; polymyxin B inhibits the growth of bacteria.
 
Formulation (per litre):
Peptone 10g
Beef extract powder 1g
D- mannitol 10g
Sodium chloride 10g
Agar 15g
Phenol red 0.025g
Final pH7.4 ± 0.2
 
How to use:
1. Suspend 46g in 1 L of distilled or deionized water, heated to boiling stirring until completely dissolved, dispensing into a flask, autoclave at 121 ? for 15min.
2. Diluted and treated samples.
 
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.