Man Rogosa Sharpe Medium

Item Code: HCM028 
Packaging: 500g
Supplement: SR0370 Li-Mupirocin
     
Usages:
For isolation, enumeration and cultivation of Lactic acid bacteria.
 
Principle:
Peptone, beef extract powder, yeast extract powder to provide a nitrogen source, vitamins, growth factors; ammonium citrate, magnesium sulfate, manganese sulfate, Tween-80 and sodium acetate to provide the training of various lactic acid bacteria growth factor, agar as a medium coagulant.
 
Formulation (per litre):
Peptone 10g
Beef extract powder 5g
Yeast extract powder 4g
Glucose 20g
Tween -80 1ml
Dipotassium hydrogen phosphate 2g
Sodium acetate 5g
Ammonium citrate 2g
Magnesium sulfate (MgSO4.7H2O) 0.2g
Manganese sulfate (MnSO4.4H2O) 0.05g
Agar 15g
Final pH 6.2 ± 0.2
 
How to use:
1. Suspend 64.3g in 1 L of distilled or deionized water, heated to boiling stirring until completely dissolved, dispensing into flask, autoclave at 121 ? for 15min.
2. Diluted and treated samples.
 
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.