Fraser Broth Base

Item Code: HCM023 
Packaging: 500g
Supplement:
SR0120( acriflavine hydrochloride, nalidixic acid, ferric ammonium citrate)
SR0130 (criflavine hydrochloride, nalidixic acid)

Usages:
For selective enrichment of Listeria spp.

Principle:
Casein digest, animal tissue enzyme digests, beef extract powder, yeast extract powder provide nitrogen, vitamins, amino acids and growth factors; sodium chloride maintains osmotic equilibrium; disodium hydrogen phosphate and potassium dihydrogen phosphate buffer agent; Esculin is fermentable sugars; lithium chloride and other antibiotics can inhibit the Gram-negative bacteria and most gram-positive bacteria.
 
Formulation?per litre ?:
Casein digest 5.0g
Animal tissue enzyme digests 5.0g
Beef extract powder 5.0g
Yeast extract powder 5.0g
NaCl 20.0g
Disodium hydrogen phosphate 12.0g
Potassium dihydrogen phosphate 1.35g
Esculin 1.0 g
Lithium chloride 3.0g
 Final pH 7.2 ± 0.2
 
How to use?
1. Suspend 28.7g in 1L of distilled water, stirring heated to boiling, autoclave at 121? for 15 minutes.
2. Diluted and treated samples.
 
Quality control?

 
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.