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Analytical Science
Fluorescence Spectrophotometers
F-7100 Fluorescence Spectrophotometer
Features:
Best-in-Class" Analytical Signal-to-Noise. 360 of S/N (P-P) and > 1,200 of S/N (RMS)
Due to its enhanced sensitivity (1.5x higher), weak signals can be detected with very low noise levels. An example of the high sensitivity analysis for fluorescein is shown. The F-7100 detected fluorescence in the order of 1x10-13 mol/L (sub-picomol) compared with a blank sample (purified water); a useful calibration was obtained in the ultra-trace range.
Industry leading lamp lifetime
Light source with 5x*¹ longer lifetime compared to conventional instruments – lifetime of 2,500 hours*² –
With the application of the new Xe lamp and the improved lamp ignition power source, both luminance and the lamp lifetime were increased.
Increased lamp lifetime reduces operating Cost-of-Ownership and instrument service time.
High-Speed Scan at 60,000 nm/min – Example of reaction tracing with a spectrum
The fast-speed scanning enables users to carry out measurements that have been difficult with conventional instruments. In this example, an isomerization process of coumarin in kerosene was traced by spectrum measurement at 2-second intervals and displayed as a 3-D time-scan spectrum. This is a new function in the F-7000.
Previously, a quick reaction which occurs within 1 minute could be measured only by using the fixed wavelength method. The F-7000 is capable of following such a quick reaction because of its fast scanning, measuring the entire wavelength range within 1 second.
FL intensity standardization to correct for fluorecence intensity variation over time and between instruments
The variations in the fluorescence intensity over time and between instruments can be corrected. Fluorescence intensity is affected by changes in lamp brightness, room temperature, optical system, etc. The fluorescence intensity of the standard sample is measured, and the sample fluorescence intensity is converted to the fluorescence intensity relative to the standard sample. This standardization is also used for the intensity comparison between different instruments, including the analysis of humic substances in environmental water (conversion to quinine sulfate), the analysis of chlorophyll in water (conversion to fluorescein), and the specified value for reagent purity (conversion to quinine sulfate).
| Sensitivity (Raman light of water) | Noise: Background: S/N 20,000 or above*3 |
| . | Noise: Peak: 1,200 or above*4 |
| Minimum sample volume | 0.6 mL (in use of standard 10 mm rectangular cell) |
| Photometric principle | Monochromatic light monitoring ratio calculation |
| Light source | 150 W xenon lamp, self-deozonating lamp house |
| Monochromator | Stigmatic concave diffraction grating: 900 lines/mm, F2.2 Brazed wavelength: Excitation side 300 nm, emission side 400 nm |
| Measuring wavelength range (on both EX and EM) | 200 to 750 nm, and zero-order light (Expandable up to 900 nm with optional detector) |
| Bandpass | Excitation side: 1, 2.5, 5, 10, 20 nm Emission side: 1, 2.5, 5, 10, 20 nm |
| Resolution | 1.0 nm (at 546.1 nm) |
| Wavelength accuracy | ±1 nm |
| Wavelength scan speed | 30, 60, 240, 1,200, 2,400, 12,000, 30,000, 60,000 nm/min |
| Wavelength drive speed | 60,000 nm/min |
| 3D measurement time | 3 min*5 |
| Working temperature | 15 to 35 °C, 25 to 80% (condensation not/humidity allowed, 70% or less at 30 °C, or higher) |
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