Extract Reagent (Total RNA Isolation Reagent)

Item code: SN003-0100

The Extract Reagent provides an efficient 3-step method to isolate the total RNA from the tissue, cultured animal and bacterial cells, blood, and serum. This unique reagent system ensures the total RNA with a high yield and good quality from samples of unlimited size. If a larger sample is required, the reagent volume can be scaled proportionally, making this reagent not only very user-friendly but also highly versatile. The RNA phenol extraction is not required, and the entire procedure can be completed in 60 minutes. The total RNA is ready for use in RT-PCR, Northern Blotting, cDNA Synthesis and Mapping.

Application
Molecular biology applications, including real-time RT-PCR, microarray analysis, next-generation sequencing (RNA-Seq), northern blotting, and cloning.

Quality Control
The quality of the Extract Reagent is tested on a lot-to-lot basis to ensure consistent product quality

Size: 100 Reactions

Sample:
Fresh tissues (Up to 50 mg)
Cultured animal cells (Up to 5X 10?)
Cultured bacterial cells (Up to 1 X 10?)
Fresh blood/frozen blood (Up to 300 μl)
Serum (Up to 100 μl)

Format:
Reagent form
Operation time: 15-20 minutes
Elution volume: 50-200 μl

Features

• Fast procedure and delivering high-quality total RNA.
• Ready-to-use RNA for high performance in any downstream application.
• Consistent RNA yield from the starting material with a small amount.
• Provide sufficient reagents and 3 steps to treat the samples.

Kit Contents