Eosin Methylene Blue Agar

Item Code: HCM044 
Packaging: 500g
 
Usages:
For separating Gram-negative enterobacteria especially coliform and faecal coliform.
 
Principle:
Peptone provides carbon and nitrogen; lactose is coliform fermentable sugars; dipotassium phosphate as a buffer; agar as culture medium coagulant; eosin beauty blue is bacteriostatic agents and pH indicator, inhibits leather Gram-positive bacteria.
 
Formulation?per litre ?:
Peptone 10g
Lactose 10g
Dipotassium hydrogen phosphate 2g
Agar 15g
Eosin Y 0.4g
Methylene 0.065g
Final pH 7.1 ± 0.2
 
How to use?
1. Suspend 37.5g in 1L of distilled water. Heat with frequent agitation and boil to completely dissolve. Distribute into flasks. Autoclave at 121? for 15 minutes.
2. Diluted and treated samples.
 
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.