Usages:
For the rapid detection and of coliforms and E. coli.
Principle:
Peptone and yeast extract powder provides carbon and nitrogen sources and traces elements; sodium chloride maintains osmotic equilibrium; agar as medium coagulant; dodecyl sulfate inhibits Gram-positive bacteria; chromogenic substrate was mixed occurrence of coliforms and E. coli enzyme corresponding specific reactions, hydrolysis of the substrate, the release of the colour groups, in a pale yellow plates coliforms appears orange-red colonies while E.coli appears blue-green colonies.
Packaging size:
1000ml
Formulation (per litre):
Peptone 15.0g
Yeast extract powder 3.0g
Sodium chloride: 5.0g
Sodium lauryl sulfate: 0.1g
Agar: 12.0g
Mixed chromogenic substrate: 6.77g
Final pH 7.0 ± 0.2
How to use:
- Suspend 41.9g of the product, adding, 1.0 L of distilled or deionized water, heated to boiling stir until completely dissolved, dispensing into the flask, 115 oC autoclaved 10minutes.
- Take 25.0g or 25.0mL of the sample with sterile procedures, added to the flask containing 225.0mL of sterile phosphate-buffered saline (or saline) , shaken thoroughly homogenized with a homogenizer or a 1:10 dilution of 1min solution, diluted 1:10 and then continue to select the appropriate serial dilutions of three, the two plates inoculated with each dilution, poured dissolved by heating and cooled to about 45 oC medium.
- Observe the results.
Quality control:
This product appears light yellow after pouring on a plate, these strains were inoculated after 36 ± 1 oC 18 ~ 24h culture growth in the following table.
Bacteria name |
Growth situation |
Features |
Escherichia coli (ATCC25922) |
Good |
Blue-green colonies |
Citrobacter (ATCC8090) |
Good |
Orange-red colonies |
Salmonella Typhimurium (CMCC50115) |
Good |
Colourless colonies |
Enterococcus faecalis (ATCC29212) |
Suppressed |
- |
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light.
Specifications: Configurable 1L of Medium.