E.coli Chromogenic Medium

Usages:
For the rapid detection and enumeration of Escherichia coli.
 
Principle:
Peptone and yeast extract powder provides carbon and nitrogen sources and traces elements; sodium chloride maintains osmotic equilibrium; agar as medium coagulant; dodecyl sulfate inhibits Gram-positive bacteria; chromogenic substrate and large intestine coli β- glucuronidase enzyme-specific reaction, hydrolysis of the substrate, the release of the colour groups produce green colonies on the light yellow plate.

Packaging size: 
1000ml
 
Formulation (per litre):
Peptone:15.0g
Yeast extract powder: 3.0g
Sodium chloride: 5.0g
Sodium lauryl sulfate:0.1g
Agar: 12.0g
Chromogenic substrate 6.5g
Final pH 7.0 ± 0.2
 
How to use:

1. Suspend 41.6g of the product, adding 1.0L of distilled or deionized water, heated to boiling stirring until completely dissolved, dispensing into the flask, autoclave at 115 ^oC for 10minutes.
2. Take 25.0g or 25.0ml of the sample with sterile procedures, added to the flask containing 225.0mL of sterile phosphate-buffered saline (or saline), shaken thoroughly homogenized with a homogenizer or a 1:10 dilution of 1min solution, diluted 1:10 and then continue to select the appropriate serial dilutions of three, the two plates inoculated with each dilution, poured dissolved by heating and cooled to about 45 ^oC medium.
3. Observe the results.

 
Quality Control
This product appears light yellow after the pouring on the plate, these strains were inoculated after 36 ± 1 ^oC 18 ~ 24h culture growth in the following table.

 
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light.