EC Medium

Usages?
For determination of faecal coliforms and E. coli confirmatory testing.
 
Principle:
Tryptone provides carbon and nitrogen sources; the 3rd bile suppresses gram-positive bacteria, especially against Gram-positive bacteria and faecal streptococci; lactose is fermentable sugars; potassium hydrogen phosphate and potassium dihydrogen phosphate as a buffer; sodium chloride maintains osmotic equilibrium.

Item code: HCM005 
Packaging size: 500g
 
Formulation (per litre):
Tryptone: 20.0g
The 3rd bile: 1.5g
Lactose: 5.0g
Dipotassium hydrogen: 4.0g
Potassium dihydrogen phosphate: 1.5g
Sodium chloride:5.0g
Final pH 6.9 ± 0.2
 
How to use:

1. Suspend 37g in 1 L of distilled water or deionized water, stirring heated to boiling until completely dissolved, packed in with a small test tube inverted tube, and autoclave at 121 ? for 15min, leave to cool to room temperature and set aside.
2. Diluted and treated sample.

 
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.