Sample: 30~100 mg of fresh animal tissue
Up to 25 mg of paraffin-embedded tissue
Format: Reagent and spin column
Operation time: within 60 minutes
Features
Ready-to-use genomic DNA for high performance in any downstream application.
Highly purified and high yield genomic DNA can be extracted from various tissue samples.
Optimized tissue lysis buffer for the efficient lysis.
Elution volume: 50~200 μl
Kit Contents
Contents
Size
Buffer DG
100 ml
Buffer BD
100 ml
Buffer W1
45 ml
Buffer W2 (add ethanol)
15 ml (60 ml)
Buffer BE
10 ml
DGT Column
50 pieces x 2
Collection Tube
50 pieces x 2
The Dual Genomic DNA Isolation Kit (Tissue) is designed to combine reagent system and spin column system. The kit could be used specifically for genomic DNA isolation from animal tissue samples. This unique reagent system ensures the total DNA with high yield and good quality from samples. The spin column system is designed to purify or concentrate DNA products which have been previously isolated with the Reagents. The entire procedure can be completed in 1 hour without phenol extraction. Purified DNA is suitable for use in PCR or other enzymatic reactions.
Applications
Gene cloning
SNP genotyping
Real time PCR
Southern blotting
Quality Control
The quality of the Dual Genomic DNA Isolation Kit (Tissue) is tested on a lot-to-lot basis to ensure consistent product quality.