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Life Science
Reagent + Column
DUAL Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus)
Item code: SN015-0100
Size: 100 Reactions
Sample:
Up to 300 μl of the whole blood
Up to 200 μl of the frozen blood
Up to 200 μl of the buffy coat
Cultured animal cells (up to 1 x 107)
Cultured bacterial cells (up to 1 x 109)
Fungus cells (up to 5 x 108)
Format: Reagent and spin column
Yield:
Up to 50 μg
Operation time: Within 60 minutes
Elution volume: 50∼200 μl
Features
- Fast, reproducible and easy processing by using reagent or spin column system.
- To isolate high quality genomic DNA.
- Isolated genomic DNA is compatible with various downstream applications.
Kit Contents
| Contents | Size |
| Buffer RL | 100 ml |
| Buffer CL | 35 ml |
| Buffer PO | 12 ml |
| Buffer BD | 45 ml |
| Buffer W1 | 45 ml |
| Buffer W2 (Add ethanol) | 15 ml (60 ml) |
| Buffer BE | 10 ml |
| DG Column | 50 pieces x 2 |
| Collection Tube | 50 pieces x 2 |
The DUAL Genomic DNA Isolation Kit (Blood/ Cultured Cell/ Fungus) is designed to combine the reagent system and spin column system. The kit could be used to isolate the genomic DNA from the whole blood, frozen blood, buffy coat, cultured animal/ bacterial cells, and fungus. This unique reagent system ensures the DNA with a high yield and good quality from the samples. The spin column system is designed to purify or concentrate DNA products which have been previously isolated with the reagents. The entire procedure can be completed in 1 hour without the phenol/ chloroform extraction. The purified DNA is suitable for use in PCR or other enzymatic reactions.
Applications
- Restriction enzyme digestion.
- Southern blotting.
- PCR amplification.
- Real-time PCR assay.
Quality Control
The quality of the DUAL Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus) is tested on a lot-to-lot basis to ensure consistent product quality.
| Specification | Kindly refer to the "Download" tab. |