Cetrimide Agar Base

Item Code: HCM047 
Packaging: 500g
Supplement: 029080 Glycerol

Usages:
For isolation and enumeration of Pseudomonas aeruginosa in water samples.
 
Principle:
Peptone provides nitrogen; glycerol carbon source; potassium and magnesium can promote green pus pigment production; hexadecyl trimethyl ammonium bromide is a selective antibacterial agent, as a quaternary ammonium salt cationic detergent may bacterial cells release nitrogen and phosphorus and suppress non-Pseudomonas aeruginosa bacteria; agar is a medium coagulant.
 
Formulation?per litre ?:
Pancreatic Digest of Peptone 20.0g
Magnesium Chloride     1.4g
Dipotassium Sulphate   10.0g
Cetrimide   0.3g
Agar 13.6g
Final pH 7.2 ± 0.2
 
How to use?
1. Suspend 45.3g in 1L of distilled water, stirring heated to boiling to completely dissolve, autoclave at 121? for 15 minutes.
2. Diluted and treated samples.
 
Quality control?
          ??

Item	The name and number of strain	Growth	Colony Color
1	Pseudomonas aeruginosa ATCC27853	Good	Yellow -green
2	Escherichia coli ATCC25922	Inhibition	--
3	Staphylococcus aureus ATCC25923	Inhibition	--

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.