Principle:
Peptone provides carbon and nitrogen sources to meet the needs of bacterial growth; sodium chloride maintains osmotic equilibrium; potassium dihydrogen phosphate and disodium hydrogen phosphate are as a buffer.
Suspend 20g of product, adding 1L of distilled or deionized water, heated to boiling stirring until completely dissolved, dispensing into flask, autoclave at 121 ? for 15min, set aside.
Diluted and treated samples.
Quality control?
Item
The name and number of strain
Growth
Colony Color
1
Salmonella typhi CMCC (B) 50071
Good
Cloudy broth
2
Salmonella typhimurium CMCC (B) 50115
Good
Cloudy broth
3
Paratyphoid Salmonella CMCC (B) 50093
Good
Cloudy broth
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.