Usages?
For determination of coliform confirmatory test for multi-tube fermentation.
Principle:
Peptone provides carbon and nitrogen sources; lactose as fermentable sugars; ox bile powder and brilliant green inhibit the non-Enterobacteriaceae.
Item Code: HCM004
Packaging Size: 500g
Formulation (per litre):
Peptone: 10g
Lactose: 10g
Ox bile powder: 20g
Brilliant green: 0.0133g
Final pH7.2 ± 0.2
How to use:
- Suspend 40g of the product, add 1 L of distilled water, stirring heated to boiling until completely dissolved, packed in a test tube with a small inverted tube, autoclave at 121oC for 15min, cool to room temperature and set aside.
- Sample handling and dilution. Slightly.
- Transplanted LST broth tube to brilliant green lactose bile broth tube by using an inoculating loop.
- The tube was placed in an incubator culture at 36 ± 1oC for 24h.
- Observe the results. Press production trachea number, check table report MPN MPN value per gram (mL) sample of coliform bacteria.
Quality control:
Quality control strains were inoculated and cultured at 36 ± 1oC for 24h, results are as follows:
Bacteria name |
Growth situation |
Gassing |
Escherichia coli ATCC25922 |
Good |
+ |
Salmonella typhimurium CMCC (B) 50115 |
Good |
- |
Staphylococcus aureus ATCC6538 |
Suppressed |
- |
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.