Blood Agar Base

Item code: HCM024 
Packaging: 500g
Supplement: Defibrinated sheep blood/rabbit blood

Usages:
For the growth of pathogenic and non-pathogenic microorganisms.
 
Principle:
Polypeptone provides carbon and nitrogen sources, vitamins and growth factors; Neisseria starch can promote the growth characteristics and enhanced hemolytic streptococci; sodium chloride to maintain osmotic balance; agar as culture medium coagulant.
 
Formulation (per liter):
Peptone: 10g
Beef extract powder:10g
Sodium chloride: 5g
Agar:15g
Final pH 7.4 ± 0.2
 
How to use:
1. Suspend 40g of the product, adding 1 L of distilled or deionized water, heated to boiling stirring until completely dissolved, dispensing into the flask, 121 ? autoclaved 15min.
2. After the medium is cooled to about 50 ?, sterile procedures, the basal medium was added per 100mL defibrinated sheep or rabbit blood 5-10mL, shake pour plate; also dispensing sterile tubes set into the slope. Spare.
3. take the bacteria to be tested fresh pure cultures inoculated crossed or coated on the plate, set the temperature of the incubator training requirements were cultured for a predetermined time.
4. Observe the results.
 
Quality control:
The following were inoculated after 36 ± 1 ? for 24h, the results are as follows:
bacteria name          bacteria No.         growth status      colony characteristics
Escherichia coli          ATCC25922        not good           hemolysis
 
Staphylococcus aureus,     ATCC6538        good               β-hemolytic
 
Beta-hemolytic streptococcus CMCC (B) 32210   good             β-hemolytic
 
Streptococcus pneumoniae   CMCC (B) 31001   good            α-hemolytic