Bismuth Sulfite Agar

Item Code: HCM059 
Packaging: 500g
 
Usages:
For selective isolation of Salmonella spp, especially for Salmonella typhi.
 
Principle:
Peptone, beef extract powder provides carbon, nitrogen, vitamins and minerals; glucose to provide energy; bismuth sulfite indicator suppresses gram-positive bacteria and coliform bacteria, but does not affect the growth of salmonella; agar as culture medium coagulant.
 
Formulation?per liter?:
Peptone 10g
Beef extract powder 5g
Dextrose 5g
Ferrous sulfate 0.3g
Disodium hydrogen phosphate 4g
Sodium sulfite  6g
Bismuth ammonium citrate  2g
Brilliant green  0.025g
Agar 15g
Final pH7.2 ± 0.2
 
How to use?
1. Suspend 47.3g in 1L of distilled water, stirring heated to boiling to completely dissolve, autoclave at 121? for 15 minutes.
2. Diluted and treated samples.
 
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.